SELECTED Journal Covers
SELECTED Manuscripts - Submitted/Under Peer Review/PrePrints
2024
(138) Liu, J., Rayes, D., Yang, M., Akbari, O.S., Fluorescent based sex-separation technique in major invasive crop pest, Drosophila suzukii. 2024.
Current publication Status: Under minor Revision @ GEN Biotechnology, Manuscript Available on bioRxiv. https://www.biorxiv.org/content/10.1101/2024.10.07.617099v1
Current publication Status: Under minor Revision @ GEN Biotechnology, Manuscript Available on bioRxiv. https://www.biorxiv.org/content/10.1101/2024.10.07.617099v1
(137) Lin, C.P., Li, H., Brogan, D.J., Wang, T., Akbari, O.S., Komives, E.A., CRISPR-RNA binding drives structural ordering that primes Cas7-11 for target cleavage. 2024
Current Publication Status: Submitted to Nucleic Acid Research; Manuscript Available on bioRxiv - https://pubmed.ncbi.nlm.nih.gov/39211128/
Current Publication Status: Submitted to Nucleic Acid Research; Manuscript Available on bioRxiv - https://pubmed.ncbi.nlm.nih.gov/39211128/
(136) Gendron, W.A.C, Raban, R., Mondal, A., Sánchez C., H.M., Smidler, A., Zilberman, D., Ilboudo, P.G., D’Alessandro, U., Marshall, J.M., Akbari, O.S., Building Regional Capacity to Implement Precision Guided Sterile Insect Technique for Malaria Control in West Africa. 2024.
Current Publication Status: Under Revision @ Scientific African.
Current Publication Status: Under Revision @ Scientific African.
(135) Overton, M.S., Guy, S.E., Chen, X., Martsul, A., Carolino, K., Akbari, O.S., Meyer, J.R., Kryazhimskiy, S., Upper Bound on the Mutational Burden Imposed by a CRISPR-Cas9 Gene Drive Element. 2024
Current Publication Status: Preparing for submission; Manuscript available on bioRxiv.
https://www.biorxiv.org/content/10.1101/2023.11.28.569142v1
Current Publication Status: Preparing for submission; Manuscript available on bioRxiv.
https://www.biorxiv.org/content/10.1101/2023.11.28.569142v1
(133) Brogan, D.J., Benetta, E.D., Wang, T., Lin, C.P., Chen, F., Li, H., Lin, C., Komives, E.A., Akbari, O.S., Engineered type III-E CRISPR-Cas effectors. 2024
Current Publication Status: Preparing Submission; Manuscript Available on bioRxiv
www.biorxiv.org/content/10.1101/2024.02.23.581838v1
Current Publication Status: Preparing Submission; Manuscript Available on bioRxiv
www.biorxiv.org/content/10.1101/2024.02.23.581838v1
(132) Brostoff, T., Kwan, J., Brogan, D., Herrmann, D., Raban, R., Akbari, O.S., Reagan, K.L., Development and evaluation of a CasRx-based SENSR assay for detection of feline coronavirus. 2024.
Current Publication Status: Under Peer Review @ Gen Biotechnology
Current Publication Status: Under Peer Review @ Gen Biotechnology
(130) Maekawa, E., Li, M., Akbari, O.S., Ohsawa, O., Dahanukar, A., Disruption of carbohydrate feeding and metabolism in Aedes aegypti mutants lacking a conserved internal sweet taste receptor. 2024
Current Publication Status: Under Peer Review @ Review Commons
Current Publication Status: Under Peer Review @ Review Commons
(129) Shankar, S., Tauxe, G.M., Spikol, E.D., Li, M., Akbari, O.S., Giraldo, D., McMeniman, C.J., Synergistic Coding of Human Odorants in the Mosquito Brain. 2024
Current Publication Status: Under Revision @ G3; Manuscript available on bioRxiv.
Current Publication Status: Under Revision @ G3; Manuscript available on bioRxiv.
(128) Clark, J.T., Nunez-Flores, R., Ejercito, J., Guda, T., Naters, W., Luy, M., Li, M., Akbari, O.S., Ray, A., Insect olfactory neurons and receptors detect DEET through widespread activation and selective inhibition. 2024
Current Publication Status: Under Revision @ Cell Reports
Current Publication Status: Under Revision @ Cell Reports
SELECTED PUBLICATIONS - Peer reviewed/Accepted/ In Press
(127) Coutinho-Abreu, I.V., Chen, F., Li, HS., Rose, N.H., Akbari, O.S., Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9. 2024
Current publication Status: Accepted and In Press @ Jove.
Current publication Status: Accepted and In Press @ Jove.
(126) Weng, S.C., Chen, F., Li1, M., Lee, S., Gerry, C., Turksoy, D.C., Akbari, O.S., Establishing a Dominant Early Larval Sex-Selection Strain in the Asian Malaria Vector Anopheles stephensi. 2024. BMC Infectious Diseases of Poverty (IDOP). doi: https://doi.org/10.1186/s40249-024-01256-7
PDF
s40249-024-01256-7.pdf
s40249-024-01256-7.pdf
(125) Wei, Z., Wang, Y., Zheng, K., Wang, Z., Liu, R., Wang, P., Li, Y., Gao, P., Akbari, O.S., Yang, X., Loss-of-function in testis-specific serine/threonine protein kinase (TSSKs) triggers male infertility in an invasive moth. 2024. Communications Biology. doi: https://doi.org/10.1038/s42003-024-06961-5.
PDF
42003_2024_article_6961.pdf
42003_2024_article_6961.pdf
(124) Liu, F., Coutinho-Abreu, I.V., Raban, R., Thuy Dan Nguyen, T., Dimas, A.R., Merriman, J.A., Akbari, O.S., Engineered Skin Microbiome Reduces Mosquito Attraction to Mice. 2024. PNAS Nexus.
8zfgj4-pgae267.pdf
Press Releases:
https://www.livescience.com/health/the-skin-microbiome-could-be-harnessed-as-mosquito-repellent-study-hints
https://newatlas.com/science/engineered-bacteria-mosquito-repellent/
https://www.iflscience.com/could-we-topple-the-planets-deadliest-animal-with-engineered-human-skin-microbes-75364
https://www.genengnews.com/topics/infectious-diseases/skin-microbiome-with-living-repellent-reduces-mosquito-attraction/
https://interestingengineering.com/science/living-mosquito-repellent-from-human-skin
https://www.the-microbiologist.com/news/engineered-human-skin-bacteria-repel-mosquitoes-for-11-days-study-shows/3759.article
https://newatlas.com/science/engineered-bacteria-mosquito-repellent/
https://www.azolifesciences.com/news/20240801/Revolutionary-Bacterial-Engineering-Reduces-Mosquito-Attraction-in-Mice.aspx
8zfgj4-pgae267.pdf
Press Releases:
https://www.livescience.com/health/the-skin-microbiome-could-be-harnessed-as-mosquito-repellent-study-hints
https://newatlas.com/science/engineered-bacteria-mosquito-repellent/
https://www.iflscience.com/could-we-topple-the-planets-deadliest-animal-with-engineered-human-skin-microbes-75364
https://www.genengnews.com/topics/infectious-diseases/skin-microbiome-with-living-repellent-reduces-mosquito-attraction/
https://interestingengineering.com/science/living-mosquito-repellent-from-human-skin
https://www.the-microbiologist.com/news/engineered-human-skin-bacteria-repel-mosquitoes-for-11-days-study-shows/3759.article
https://newatlas.com/science/engineered-bacteria-mosquito-repellent/
https://www.azolifesciences.com/news/20240801/Revolutionary-Bacterial-Engineering-Reduces-Mosquito-Attraction-in-Mice.aspx
(123) Smidler, A.L., Apte, R.A., Pai1, J.J., Chow, M.L., Chen, S., Mondal, A., Sánchez C, H.M., Antoshechkin, I., Marshall, J.M., Akbari, O.S., Eliminating Malaria Vectors with Precision Guided Sterile Males. 2024. PNAS. doi: www.pnas.org/doi/10.1073/pnas.2312456121
PDF:
apte-et-al-2024-eliminating-malaria-vectors-with-precision-guided-sterile-males.pdf
PNAS Primer Article:
rasgon-2024-precision-guided-tools-for-malaria-control.pdf
Press Releases:
https://www.reddit.com/r/science/comments/1doe126/researchers_have_used_crispr_to_create_mosquitoes/
https://www.news-medical.net/news/20240626/CRISPR-based-genetic-technique-eradicates-malaria-mosquitoes-with-over-9925-efficiency.aspx
https://singularityhub.com/2024/07/08/gene-drives-shown-to-work-in-wild-plants-they-could-wipe-out-weeds/
PDF:
apte-et-al-2024-eliminating-malaria-vectors-with-precision-guided-sterile-males.pdf
PNAS Primer Article:
rasgon-2024-precision-guided-tools-for-malaria-control.pdf
Press Releases:
https://www.reddit.com/r/science/comments/1doe126/researchers_have_used_crispr_to_create_mosquitoes/
https://www.news-medical.net/news/20240626/CRISPR-based-genetic-technique-eradicates-malaria-mosquitoes-with-over-9925-efficiency.aspx
https://singularityhub.com/2024/07/08/gene-drives-shown-to-work-in-wild-plants-they-could-wipe-out-weeds/
(122) Rouyar, A., Patil, A.A., Norena, M.L., Li, M., Coutinho-Abreu, I.V, Akbari, O.S., Riffell, J.A., Transgenic line for characterizing GABA-receptor expression to study the neural basis of olfaction in the yellow-fever mosquito. 2024. Frontiers in Physiology. doi: https://doi.org/10.3389/fphys.2024.1381164
PDF
fphys-15-1381164.pdf
fphys-15-1381164.pdf
(121) Nadya Urakova, Renuka E. Joseph, Allyn Huntsinger, Vanessa M. Macias, Matthew J. Jones, Leah T. Sigle, Ming Li, Omar S. Akbari, Zhiyong Xi, Konstantinos Lymperopoulos, Richard T Sayre, Elisabeth A. McGraw, Jason L. Rasgon. Alpha-mannosidase-2 modulates arbovirus infection in a pathogen- and Wolbachia-specific manner in Aedes aegypti mosquitoes. 2024. Insect Molecular Biology. doi: https://doi.org/10.1111/imb.12904
PDF:
insect_molecular_biology_-_2024_-_urakova_-_alpha_e2_80_90mannosidase_e2_80_902_modulates_arbovirus_infection_in_a_pathogen_e2_80_90_and.pdf
PDF:
insect_molecular_biology_-_2024_-_urakova_-_alpha_e2_80_90mannosidase_e2_80_902_modulates_arbovirus_infection_in_a_pathogen_e2_80_90_and.pdf
(120) Liu, , J., Rayes, D., Akbari, O.S., A fluorescent sex-sorting technique for insects with the demonstration in Drosophila melanogaster. 2024. Gen Biotechnology. doi: https://doi.org/10.1089/genbio.2023.0041.
(119) Coutinho-Abreu, I.V., Jamshidi, O., Raban, R., Atabakhsh, K., Merriman, J.A., Akbari, O.S., Identification of human skin microbiome odorants that manipulate mosquito landing behavior - towards engineering a repellent skin flora. 2024. Nature Scientific Reports. doi https://doi.org/10.1038/s41598-023-50182-5
PDF:
s41598-023-50182-5.pdf
Press Releases:
New Scientist - Your skin microbiome may affect how attractive you are to mosquitoes
PDF:
s41598-023-50182-5.pdf
Press Releases:
New Scientist - Your skin microbiome may affect how attractive you are to mosquitoes
(118) Bui, M., Raban, R., Akbari, O.S., Precise Genome Editing of the Asian Citrus Psyllid. 2024. Citrograph.
Link to PDF:
m.bui-crb-citrograph-mag-q1-winter-2024.pdf
Link to PDF:
m.bui-crb-citrograph-mag-q1-winter-2024.pdf
2023
(117) Weng, SC., Antoshechkin, I., Marois, E., Akbari, O.S., Efficient Sex Separation by Exploiting Differential Alternative Splicing of a Dominant Marker in Aedes aegypti. 2023. Plos Genetics. doi: https://doi.org/10.1371/journal.pgen.1011065
(116) Dalla Benetta, E., López-Denman, A., Li, H.H., Masri, R.A., Brogan, D.J., Bui, M., Yang, T., Li, M., Dunn, M., Klein, M.J., Jackson, S., Catalan, K., Blasdell, K.R., Tng, P., Antoshechkin, I., Alphey, L.S., Paradkar, P.N., Akbari, O.S., Engineered Antiviral Sensor Targets Infected Mosquitoes. 2023. The CRISPR Journal. doi: ttps://doi.org/10.1089/crispr.2023.0056
Press Release(s)
https://sciworthy.com/scientists-tackle-mosquito-borne-viruses-with-crispr/
Press Release(s)
https://sciworthy.com/scientists-tackle-mosquito-borne-viruses-with-crispr/
(115) Davydova, S., Liu, J., Kandul, N.P., Braswell, E., Akbari, O.S., Meccariello, A., Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata. 2023
Nature Scientific Reports. doi. https://doi.org/10.1038/s41598-023-47276-5 davydova_et_al-2023-scientific_reports.pdf
Press Release(s)
https://www.imperial.ac.uk/news/249553/pandemic-learnings-pest-control-developments-news/
Nature Scientific Reports. doi. https://doi.org/10.1038/s41598-023-47276-5 davydova_et_al-2023-scientific_reports.pdf
Press Release(s)
https://www.imperial.ac.uk/news/249553/pandemic-learnings-pest-control-developments-news/
(114) Weng, SC, Masri, R.A., Akbari, O.S., Advances and Challenges in Synthetic Biology for Mosquito Control. 2023. Trends In Parasitology. doi: https://doi.org/10.1016/j.pt.2023.11.001 1-s2.0-s1471492223002817-main.pdf
(112) Chaverra-Rodriguez, D., Bui, M., Rasgon, J.L., Akbari, O.S., CRISPR/Cas9-mediated mutagenesis of the Asian Citrus Psyllid, Diaphorina citri. Gen Biotechnology. 2023. doi: https://doi.org/10.1089/genbio.2023.0022
(110) Lutrat, C., Burckbuchler, M., Proveti Olmo, R., Beugnon, R., Fontaine, A., Akbari, O.S. , Argiles, R., Baldet, T, Bouyer, J., Marois, E., Combining two Genetic Sexing Strains allows sorting of non-transgenic males for Aedes genetic control. Communications Biology. 2023. doi. https://doi.org/10.1038/s42003-023-05030-7
Press releases
Fluorescent sexing strains to facilitate the Sterile Insect Technique in the dengue and tiger mosquitoeshttps://bioengineeringcommunity.nature.com/posts/fluorescent-sexing-strains-to-facilitate-the-sterile-insect-technique-in-the-dengue-and-tiger-mosquitoes
Press releases
Fluorescent sexing strains to facilitate the Sterile Insect Technique in the dengue and tiger mosquitoeshttps://bioengineeringcommunity.nature.com/posts/fluorescent-sexing-strains-to-facilitate-the-sterile-insect-technique-in-the-dengue-and-tiger-mosquitoes
(109) Smidler, A.L., Pai, J.J., Apte, R.A., Sánchez C., H.M., Corder, R.M., Gutiérrez, E.J., Thakre, N., Antoshechkin, I., Marshall, J.M., Akbari, O.S., A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiae. 2023. Science Advances. doi. https://doi.org/10.1126/sciadv.ade890
Press Releases:
New Genetic Technology Developed to Halt Malaria-Spreading Mosquitoes
UC San Diego using genetically modified mosquitoes to take on malaria
CRISPR’d Mosquitoes With All-Male Offspring Could Help Eradicate Malaria
Every minute, a child under 5 dies from malaria. Here’s how gene editing mosquitoes could quickly dramatically suppress disease-carrying populations
Halting Malaria in Its Tracks
Could a new gene-editing technique be a major breakthrough in the battle against malaria?
Press Releases:
New Genetic Technology Developed to Halt Malaria-Spreading Mosquitoes
UC San Diego using genetically modified mosquitoes to take on malaria
CRISPR’d Mosquitoes With All-Male Offspring Could Help Eradicate Malaria
Every minute, a child under 5 dies from malaria. Here’s how gene editing mosquitoes could quickly dramatically suppress disease-carrying populations
Halting Malaria in Its Tracks
Could a new gene-editing technique be a major breakthrough in the battle against malaria?
(101) Taitingfong, R.I., Triplett, C., Vásquez, V.N., Rajagopalan, R., Raban, R., Roberts, A., Terradas, G., Baumgartner, B., Emerson, C., Gould, F., Okumu, F., Schairer, C.E., Bossin, H., Buchman, L., Campbell, K.J., Clark, A., Delborne, J., Esvelt, K., Fisher, J., Friedman, R.M., Gronvall, G., Gurfield, N., Heitman, E., Kofler, N., Kuiken, T., Kuzma, J., Manrique-Saide, P., Marshall, J.M., Montague, M., Morrison, A., Opesen, C.C., Phelan, R., Piaggio, A., Quemada, H., Rudenko, L., Sawadogo, N., Smith, R., Tuten, H., Ullah, A., Vorsino, A., Windbichler, N., Akbari, O.S., Long, K., Lavery, J., Weiss Evans, S., Tountas, K., Bloss, C.S. Exploring the Value of a Global Gene Drive Project Registry. 2023. doi. https://doi.org/10.1038/s41587-022-01591-w
Press Release(s)
Experts from 14 Nations Discuss Global Gene Drive Project Registry
Press Release(s)
Experts from 14 Nations Discuss Global Gene Drive Project Registry
2022
(96) Raban, R., Gendron, W., Akbari, O.S., A perspective on the expansion of the genetic technologies to support the control of neglected vector-borne diseases and conservation. 2022. Frontiers in Tropical Diseases. doi: https://doi.org/10.3389/fitd.2022.999273
(95) Kandul, N.P., Liu, J., Buchman, A., Shriner, I.C., Corder, R.M., Warsinger-Pepe, N., Yang, T., Yadav, A.K., Scott,M.J., Marshall, J.M., Akbari, O.S., Precision Guided Sterile Males Suppress Populations of an Invasive Crop Pest. 2022. Gen Biotechnology. doi.
https://doi.org/10.1089/genbio.2022.0019
Press Release(s)
CRISPR-based Technology Targets Global Crop Pest
Environment Friendly CRISPR Technology Could Check Crop Pests
Tweaking crops to prevent disease and reduce pesticide use — a CRISPR solution - Genetic Literacy Project
PODCAST -Talking Biotech with Dr. Kevin Folta - Precision Insect Control with Gene Editing
How CRISPR could help save crops from devastation caused by pests
Agtech startup Agragene relocates from San Diego to St. Louis, bringing with it thousands of fruit flies
https://doi.org/10.1089/genbio.2022.0019
Press Release(s)
CRISPR-based Technology Targets Global Crop Pest
Environment Friendly CRISPR Technology Could Check Crop Pests
Tweaking crops to prevent disease and reduce pesticide use — a CRISPR solution - Genetic Literacy Project
PODCAST -Talking Biotech with Dr. Kevin Folta - Precision Insect Control with Gene Editing
How CRISPR could help save crops from devastation caused by pests
Agtech startup Agragene relocates from San Diego to St. Louis, bringing with it thousands of fruit flies
(94) Brogan, D.J, and Akbari, O.S., CRISPR Diagnostics: Advances Towards the Point-of-Care. 2022. ACS Biochemistry. doi. https//doi.org/10.1021/acs.biochem.2c00051
(90) Raban, R., and Akbari, O.S. 2nd edition of the book Transgenic Insects: Techniques and Applications. Chapter 3. An Introduction to the Molecular Genetics of Gene Drives and Thoughts on Their Gradual Transition to Field Use. 2022. doi. https://doi.org/10.1079/9781800621176.0008
2021
(89) Liu, W-L., Hsu, C-W., Chan, S-P., Yen, P-S., Su, M.P., Li, J-C., Li, H-H., Cheng, L., Tang, C-K., Ko, S-H., Tsai, H-K., Tsai, Z.T-Y., Akbari, O.S., Failoux, A-B., Chen, C-H., Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus. 2021. Nature Scientific Reports. doi https://doi.org/10.1038/s41598-021-03229-4
(88) Gamez, S., Chaverra-Rodriguez, D., Buchman, A., Kandul, N.P., Sanchez, S.C.M., Bennett, J.B., Sanchez, H.M., Yang, T., Antoshechkin, I., Duque, J.E., Papathanos, P.A., Marshall, J.M., Akbari, O.S., Exploiting a Y Chromosome linked Cas9 for Sex Selection and Gene drive. 2021. Nature Communications. doi https://doi.org/10.1038/s41467-021-27333-1
Supplementary Information pdf
Supplementary Information pdf
(87) Brogan,D.J., Chaverra-Rodriguez, D., Lin, C.P., Smidler, A.L., Yang, T., Alcantara, L.M., Antoshechkin, I., Liu, J., Raban, R.R., Belda-Ferre, P., Knight, R., Komives, E.A., Akbari, O.S., Development of a Rapid and Sensitive CasRx-based Diagnostic Assay for SARS-CoV2. 2021. ACS SENSORS. doi https://doi.org/10.1021/acssensors.1c01088.
Supplementary Information pdf
Press Release(s)
Supplementary Information pdf
Press Release(s)
- Panel Discusses Campus Initiatives and Research Labs Work during COVID-19
- UC San Diego: A Deep Look Into The New World Of COVID-19 Innovation
- Researchers Develop CRISPR-based Rapid Diagnostic Tool for SARS-CoV-2
- CRISPR-based diagnostics: expanding the toolbox against COVID-19
- UCSD Scientists Develop SENSR Coronavirus Detection System
- UCSD Developing SARS-CoV-2 At-Home, Molecular Test Leveraging CRISPR, Isothermal Amplification
(86) Parks, S.C., Nguyen, C., Nasrolahi, S., Juncaj, D., Lu, D.,, Ramaswamy, R., Buchman, A., . Akbari, O.S., Yamanaka, N., Boulanger, M.J., Dillman, A.R., Parasitic nematodes secrete fatty acid- and retinol-binding proteins to compromise host immunity by interfering with host lipid signaling pathways. 2021.
PLoS Pathog. doi: 10.1371/journal.ppat.1010027.
PLoS Pathog. doi: 10.1371/journal.ppat.1010027.
(83) Kandul, N.P., Belikoff, E.J., Liu, J., Buchman, A., Li, F., Yamamoto, A., Yang, T., Shriner, I., Scott, M.J., Akbari, O.S., Genetically Encoded CRISPR components Yield Efficient Gene Editing in the Invasive Pest, Drosophila suzukii. 2021. CRISPR Journal. doi https://doi.org/10.1089/crispr.2021.0032
Press Release(s)
Spotlight on Genetic Design in a Spotted Wing Crop Killer
Press Release(s)
Spotlight on Genetic Design in a Spotted Wing Crop Killer
(82) Chaverra-Rodriguez, D., Duque, J.E., Akbari, O.S., CRISPR-Cas based insect genetic control technologies and its applications for Chagas disease. Tecnologías de control genético de insectos basadas en CRISPR-Cas y su aplicación en la enfermedad de Chagas. 2021. Book Chapter for Vectores. Invited.
PDF of entire book here.
PDF of entire book here.
(80) Ming Li, Ting Yang, Michelle Bui, Stephanie Gamez, Tyler Wise, Nikolay P. Kandul, Junru Liu, Lenissa Alcantara, Haena Lee, Jyotheeswara R. Edula, Robyn Raban, Yinpeng Zhan, Yijin Wang, Nick DeBeaubien, Jieyan Chen, Hector M. Sanchez C., Jared B. Bennett, Igor Antoshechkin, Craig Montell, John M. Marshall, and Omar S. Akbari.
Suppressing Mosquitoes with Precision Guided Sterile Males. 2021. Nature Communications. doi https://doi.org/10.1038/s41467-021-25421-w
Supplementary Information pdf
Press Release(s) (>25 News outlets)
Suppressing Mosquitoes with Precision Guided Sterile Males. 2021. Nature Communications. doi https://doi.org/10.1038/s41467-021-25421-w
Supplementary Information pdf
Press Release(s) (>25 News outlets)
- Scientific American - First Genetically Modified Mosquitoes Released in U.S. Are Hatching Now
- LA TIMES: COVID isn’t spread by mosquitoes. But the next pandemic might be
- KCRW Los Angeles - NPR - Noticed more mosquitos lately? Here’s how to prevent infestations and stay safe
- www.Genengnews.com -Mosquitoes Sterilized by CRISPR Powered Precision System
- www.sciencedaily.com - New technology designed to genetically control disease-spreading mosquitoes
- New precision-guided sterile insect technique designed to control disease-spreading mosquitoes
- Can we genetically control the world’s deadliest animal?
- www.inovativegenomics.org - New Technology Designed to Genetically Control Disease-Spreading Mosquitoes
- Wolbachia to the rescue – reducing dengue just like a COVID-19 vaccine
- Republicworld.com - Australian Study Finds Bacteria Effective Against Dengue-carrying Mosquitoes
- CRISPR MEDICINE NEWS - CRISPR Provides New Hope for Control of Mosquito-Borne Viral Diseases
- CRISPR Technology Can Eliminate Disease-Spreading Mosquitoes
- You Have No Idea How Good Mosquitoes Are at Smelling Us
- Brazil’s Mosquito Factory is Supposed to Curb Dengue. There's A Better Way. https://www.asimov.press/p/mosquito-factory
(76) Buchman, A., Shriner, I., Yang, T., Liu, J., Antoshechkin, I., Marshall, J.M., Perry, M.W., Akbari, O.S.* Engineered Reproductively Isolated Species Drive Reversible Population Replacement. 2021. Nature Communications. DOI: 10.1038/s41467-021-23531-z.
Supplementary Information PDF
Press Release(s)
Supplementary Information PDF
Press Release(s)
- Synthetic SPECIES developed for use as a confinable gene drive
- Gene tech to prevent crossbreeding could safely harness the power of gene drives - The Science Advisory Board
- Scientists develop new gene drive with a built-in genetic barrier
- Scientists design new gene drive to stop the transmission of devastating diseases
- Harnessing extreme underdominance to engineer a synthetic species - Behind the Paper - Nature Bioengineering.
- The Power of Gene Drives: Altering Species Without Losing Control
- Video: Here’s how synthetic flies could become a major tool in the quest to control insect-borne diseases
(73) Benetta, E.D., Akbari, O.S., Ferree, P.M. Mechanistically Comparing Reproductive Manipulations Caused by Selfish Chromosomes and Bacterial Symbionts. 2021. Heredity. PMID: 33649572DOI: 10.1038/s41437-021-00410-3
(72) Sun, R., Brogan, D., Buchman, A., Yang, T., Akbari, O.S. Ubiquitous and tissue-specific RNA targeting in Drosophila melanogaster using CRISPR/CasRx. 2021. J Vis Exp. 2021 Feb 5;(168). doi: 10.3791/62154.
2020
(70) Kanya C. Long, Luke Alphey, Cinnamon S. Bloss, Karl J. Campbell, Jackson Champer, Chun-Hong Chen, Amit Choudhary, George M. Church, James P. Collins, Kimberly L. Cooper Jason A. Delborne, Owain R Edwards, Claudia I. Emerson, Kevin Esvelt, Sam Weiss Evans, Robert M. Friedman, Valentino M. Gantz, Fred Gould, Sarah Hartley, Elizabeth Heitman, Janet Hemingway, Hirotaka Kanuka, Jennifer Kuzma, Jim V. Lavery, Yoosook Lee, Jeantine E. Lunshof, John M. Marshall, Philipp W. Messer, Craig Montell, Kenneth A. Oye, Megan J. Palmer, Philippos Aris Papathanos, Prasad N. Paradkar, Antoinette J. Piaggio, Jason L. Rasgon, Gordana Rašić, Larisa Rudenko, J. Royden Saah, Maxwell J. Scott, Jolene T. Sutton, Adam E. Vorsino, Omar S. Akbari†, Core commitments for field trials of gene drive organisms. 2020. Science. DOI: 10.1126/science.abd1908
Link to Supplemental File PDF
Summary: http://science.sciencemag.org/cgi/content/summary/370/6523/1417?ijkey=KnRWjndPVB0Rs&keytype=ref&siteid=sci
Reprint: http://science.sciencemag.org/cgi/rapidpdf/370/6523/1417?ijkey=KnRWjndPVB0Rs&keytype=ref&siteid=sci
Full text: http://science.sciencemag.org/cgi/content/full/370/6523/1417?ijkey=KnRWjndPVB0Rs&keytype=ref&siteid=sci
CLICK HERE to Become a Signatory to these Core Commitments.
Press Releases:
Link to Supplemental File PDF
Summary: http://science.sciencemag.org/cgi/content/summary/370/6523/1417?ijkey=KnRWjndPVB0Rs&keytype=ref&siteid=sci
Reprint: http://science.sciencemag.org/cgi/rapidpdf/370/6523/1417?ijkey=KnRWjndPVB0Rs&keytype=ref&siteid=sci
Full text: http://science.sciencemag.org/cgi/content/full/370/6523/1417?ijkey=KnRWjndPVB0Rs&keytype=ref&siteid=sci
CLICK HERE to Become a Signatory to these Core Commitments.
Press Releases:
- Scientists Set a Path for Field Trials of Gene Drive Organisms
- Science Papers Present Updated Rhesus Macaque Genome Assembly, Responsible Use of Gene Drive Technology
- Over 40 Scientists Set Path for Field Trials of Gene Drive Organisms -
- Zika 5 years later: Still much to learn as ‘likely’ future outbreak looms - Healio
- Genetically Engineered Organisms: Path Set for Field Trials of Gene Drive Organisms
(68) Alphey, L.S, Crisanti, A., Randazzo, F., Akbari, O.S. Standardizing the Definition of Gene Drive. 2020. PNAS. doi. https://doi.org/10.1073/pnas.2020417117
Supplementary Table 1.
CLICK HERE to Become a Signatory to these Definitions
Supplementary Table 1.
CLICK HERE to Become a Signatory to these Definitions
(67) Navarro-Payá, D., Flis, I., Anderson, M.A.E., Hawes, P., Li, M., Akbari, O.S., Basu, S., and Alphey, L., Targeting female flight for genetic control of mosquitoes. 2020. Plos Neglected Tropical Diseases. doi. https://doi.org/10.1371/journal.pntd.0008876
Press Releases(s)
Flightless female mosquitoes generated at Pirbright could aid disease control
Press Releases(s)
Flightless female mosquitoes generated at Pirbright could aid disease control
(64) Bulger, E.A., Xu,X-R, Gantz, V.M., Klanseck, C., Heimler, S.R., Klanseck, C., Auradkar, A., Bennett, J.B., Miller, L.A., Leahy, S., Juste, S.S., Buchman, A., Akbari, O.S., Marshall, J.M., Bier, E., Active-genetic neutralizing elements for halting or deleting gene-drives. (2020). Molecular Cell.
doi:https://doi.org/10.1016/j.molcel.2020.09.003
Press Releases:
doi:https://doi.org/10.1016/j.molcel.2020.09.003
Press Releases:
(62) Chaverra-Rodriguez, D., Dalla Benetta, E., Heu, C.C., Rasgon, J., Ferree, P.M., Akbari, O.S., Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR-Cas9 ribonucleoprotein. (2020). Insect Molecular Biology. DOI: https://doi.org/10.1111/imb.12663
Awarded Biannual best paper prize by Insect Molecular Biology
Awarded Biannual best paper prize by Insect Molecular Biology
(61) Raban, R., Akbari, O.S. A day in the life of a mosquito insectary team: pushing closer to solutions for mosquito-borne diseases. (2020). Nature Lab Animal DOI: https://doi.org/10.1038/s41684-020-0617-y
(59) Cheung, C., S Gamez, S.,Carballar-Lejarazú, R., Ferman, V., Vasquez, V., Terradas, G., Schairer, C.E., Bier, E., Marshall, J.M., James, A.A., Akbari, O.S., Bloss, C.S., Translating Gene Drive Science to Promote Linguistic Diversity in Community and Stakeholder Engagement. (2020). Global Public Health, DOI: 10.1080/17441692.2020.1779328
(58) Bui, M., Li, M., Raban, R.R., Liu, N., Akbari, O.S.*, Embryo microinjection techniques for efficient site-specific mutagenesis in Culex quinquefasciatus. (2020). Journal of Visualized Experiments (JOVE). DOI: doi:10.3791/61375
Video Link:
https://www.jove.com/t/61375/embryo-microinjection-techniques-for-efficient-site-specific?status=a63381k
Video Link:
https://www.jove.com/t/61375/embryo-microinjection-techniques-for-efficient-site-specific?status=a63381k
(53) Ming Li, Ting Yang, Nikolai Kandul, Michelle Bui, Stephanie Gamez, Robyn Raban, Jared Bennett, Héctor M. Sánchez C., Gregory C. Lanzaro, Hanno Schmidt, Yoosook Lee, John M. Marshall, and Omar S. Akbari*. Development of a Confinable Gene-Drive System in the Human Disease Vector, Aedes aegypti. (2020). eLife. DOI: https://doi.org/10.7554/eLife.51701
Nature Lab Animal Research Highlight by Ellen P. Nuff (https://doi.org/10.1038/s41684-020-0490-8). pdf
Press Release(s):
Nature Lab Animal Research Highlight by Ellen P. Nuff (https://doi.org/10.1038/s41684-020-0490-8). pdf
Press Release(s):
- By Emily Packer, eLIFE Sciences
- Scientists develop first CRISPR-based gene drives for controlling dengue-carrying mosquitoes
- Zika 5 years later: Still much to learn as ‘likely’ future outbreak looms
(52) Anna Buchman, Stephanie Gamez, Ming Li, Igor Antoshechkin, Shin-Hang Lee, Shin-Wei Wang, Chun-Hong Chen, Melissa J. Klein, Jean-Bernard Duchemin, James E. Crowe, Jr., Prasad N. Paradkar*, and Omar S. Akbari*, Broad Dengue Neutralization in Mosquitoes Expressing an Engineered Antibody. (2020). Plos Pathogens. doi.org/10.1371/journal.ppat.1008103
Nature Lab Animal Research Highlight by Ellen P. Nuff (https://doi.org/10.1038/s41684-020-0490-8). pdf
Press Releases (>50 news articles below are some)
By Kelly Servick, Science Magazine, January 16, 2020 By Genelle Weule, Genetic Literacy Project, January 29, 2020 CNet in Spanish, February, 2nd 2020 carhoy.com, In Spanish, February 6th, 2020 La Nación La Arena The daily Star, January 24th, 2019 By Peter Rejcek, Singularity Hub, February 02, 2020 By City News Service, 10news, January 16, 2020 By EurekAlert!, January 16, 2020 By City News Service, Fox5, January 16, 2020 By Chris Jennewein, Times of San Diego, January 16, 2020 By Science Daily, January 16, 2020 By Nick Larvars, New Atlas, January 16, 2020 By Bonnie Burton, cnet, January 17, 2020 By James Patterson, tunisiesoir, January 18, 2020 By Jeevan Biswas, ibtimes, January 20, 2020 By Mario Aguilera, UC San Diego, January 23, 2020 By Ronald Bailey, Reason, January 24, 2020 By Brenda Gregorio-Nieto, NBC San Diego, January 26, 2020By Kevin Stone - The Heartland Institute
Press Releases (>50 news articles below are some)
By Kelly Servick, Science Magazine, January 16, 2020 By Genelle Weule, Genetic Literacy Project, January 29, 2020 CNet in Spanish, February, 2nd 2020 carhoy.com, In Spanish, February 6th, 2020 La Nación La Arena The daily Star, January 24th, 2019 By Peter Rejcek, Singularity Hub, February 02, 2020 By City News Service, 10news, January 16, 2020 By EurekAlert!, January 16, 2020 By City News Service, Fox5, January 16, 2020 By Chris Jennewein, Times of San Diego, January 16, 2020 By Science Daily, January 16, 2020 By Nick Larvars, New Atlas, January 16, 2020 By Bonnie Burton, cnet, January 17, 2020 By James Patterson, tunisiesoir, January 18, 2020 By Jeevan Biswas, ibtimes, January 20, 2020 By Mario Aguilera, UC San Diego, January 23, 2020 By Ronald Bailey, Reason, January 24, 2020 By Brenda Gregorio-Nieto, NBC San Diego, January 26, 2020By Kevin Stone - The Heartland Institute
(48) Gamez, S., Antoshechkin, I., Mendez-Sanchez, S.C., Akbari, O.S.* The Developmental Transcriptome of Ae. albopictus, a Major Worldwide Human Disease Vector. (2020). G3. Genes, Genomes, Genetics. DOI: https://doi.org/10.1534/g3.119.401006. pdf
Supplementary Data
Supplementary Data
2019
(47) Chloé Lahondère, Clément Vinauger, Ryo P. Okubo, Gabriella Wolff, Omar S. Akbari, and Jeffrey A. Riffell,* The olfactory basis of orchid pollination by mosquitoes. (2019). PNAS. Dec 23. pii: 201910589. doi: 10.1073/pnas.1910589117.
Press Releases:
News Medical Life Sciences Phys.org, by James Urton Technology Networks, Jan 22, 2020 James Urton and Kendall Daniels
(45) Akbari, O.S*. Using CRISPR to Combat Human Disease Vectors. (2019). National Academy of Engineering. The Bridge. pdf.
PDF to the Full Book.
PDF to the Full Book.
(43) John M. Marshall*, Robyn Raban, Nikolay P. Kandul, Jyotheeswara R. Edula, Tomás León, and Omar S. Akbari*. Winning the tug-of-war between effector gene design and pathogen evolution in vector population replacement strategies. (2019). Frontiers in Genetics. doi: 10.3389/fgene.2019.01072
(41) Richard Feachem, Ingrid Chen, Omar S. Akbari, Amelia Bertozzi-Villa, Samir Bhatt, Fred Binka, Maciej Boni, Caroline Buckee, Joseph Dieleman, Arjen Dondorp, Alex Eapen, Neelam Sekhri Feachem, Scott Filler, Peter Gething, Roly Gosling, Annie Haakenstad, ; Kelly Harvard, ; Arian Hatefi, ; Jimee Hwang, Dean Jamison, Kate Jones, Corine Karema, Richard Nchabi Kamwi, Altaf Lal, PhD; Erika Larson, Cynthia Lee, Margaret Lees, Neil Lobo, Angela Micah, Bruno Moonen, Gretchen Newby, Xiao Ning, Muhammad Pate, Martha Quiñones, Michelle Roh, Ben Rolfe, Dennis Shanks, Balbir Singh, Kenneth Staley, James Tulloch, MJennifer Wegbreit, Hyun Ju Woo, Winnie Mpanju-Shumbusho; LANCET COMMISSION ON MALARIA ERADICATION Malaria Eradication. Within a Generation: Ambitious, Achievable and Necessary. (2019). http://dx.doi.org/10.1016/S0140-6736(19)31139-0.
Lancet Webpage dedicated to the report here.
Press Releases:
It's 'Ambitious' — But Possible — to Eradicate Malaria by 2050, According to a Landmark Report
By Jamie Ducharme, Time Magazine, September 9, 2019 By Kate Kelland, Weekly Times (Australia), September 9, 2019 By Press Association, September 9, 2019 News 18 (India), September 9, 2019
Official Press release
List of all the press coverage here. pdf
Lancet Webpage dedicated to the report here.
Press Releases:
It's 'Ambitious' — But Possible — to Eradicate Malaria by 2050, According to a Landmark Report
By Jamie Ducharme, Time Magazine, September 9, 2019 By Kate Kelland, Weekly Times (Australia), September 9, 2019 By Press Association, September 9, 2019 News 18 (India), September 9, 2019
Official Press release
- http://malariaeradicationcommission.com/sites/default/files/press-release-lancet-commission-on-malaria-eradication.pdf. Featured on MEI’s homepage.
List of all the press coverage here. pdf
(40) Vinauger, C., Van Breugel, F., Locke, L., Tobin, K., Dickinson, M., Fairhall, A., Akbari, O.S., Riffell, J., Visual-olfactory integration in the human disease vector mosquito, Aedes aegypti. (2019). Current Biology. DOI: :https://doi.org/10.1016/j.cub.2019.06.043
Press Release(s):
Press Release(s):
- UW News (http://www.washington.edu/news/2019/07/18/mosquito-sensory-integration/). PDF HERE
- Science Daily (https://www.sciencedaily.com/releases/2019/07/190718112528.htm)
(39) Bui, M., Shyong, J., Lutz, E.K., Yang, T., Li, M., Truong, K., Arvidson,R., Buchman,A., Riffell, J.A.*, Akbari, O.S.* Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems. (2019). BMC Neuroscience. 2019 Jun 17;20(1):27. doi: 10.1186/s12868-019-0511-y. PMID: 31208328
See gCamp in Action in Ae. aegypti:
Supplemental Video 1 - Adult Mouthparts - Link
Supplemental Video 2 - Adult Antennae - Link
Supplemental Video 3 -Adult Compound Eye - Link
Supplemental Video 4 - Whole Larvae - Link
Supplemental Video 5 - Larval Brain - Link
See gCamp in Action in Ae. aegypti:
Supplemental Video 1 - Adult Mouthparts - Link
Supplemental Video 2 - Adult Antennae - Link
Supplemental Video 3 -Adult Compound Eye - Link
Supplemental Video 4 - Whole Larvae - Link
Supplemental Video 5 - Larval Brain - Link
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(38) Dalla Benetta, E., Akbari, O.S., Ferree, P.M., Sequence Expression from Supernumerary B Chromosomes: Function or Fluff? (2019). Genes, 10, 123; doi:10.3390/genes10020123
(36) Kandul, N.P, Liu, J., Sanchez C., H.M.,Wu, S.L., Marshall, J.M., Akbari,O.S.*, Transforming Insect Population Control with Precision Guided Sterile Males with Demonstration in Flies. Nature Communications. volume 10, Article number: 84 (2019) DOI: https://doi.org/10.1038/s41467-018-07964-7
Supplementary Information. pdf
Press Releases:
Supplementary Information. pdf
Press Releases:
- New CRISPR-based technology developed to control pests with precision-guided genetics.
- Precision-Guided Sterile Insect Technique. IGTRCN
- Broadcasted Live on Local TV in San Diego - NBC 7
- https://www.nbcsandiego.com/on-air/as-seen-on/Local-Researchers-Develop-Insect-Gene-Editing-Tool_San-Diego-504137652.html
- https://www.goodfruit.com/genetic-control-for-spotted-wing-drosophila/
- Scientists Are Gene-Editing Flies to Fight Crop Damage. Wired.
2018
(32) Buchman, A.B, Ivy, T., Marshall, J.M., Akbari, O.S.*, Hay B.A.* Engineered reciprocal chromosome translocations drive high threshold, reversible population replacement in Drosophila. ACS Synthetic Biology. (2018) doi: PMID: 29608276. DOI: 10.1021/acssynbio.7b00451 . pdf
Supporting Information. pdf
Press Release(s):
Supporting Information. pdf
Press Release(s):
(30) Buchman, A.B., Marshall, J.M, Ostrovski, D., Yang, T., Akbari O.S.*, Synthetically Engineered Medea Gene Drive System in the Worldwide Crop Pest, D. suzukii. (2018) Proceedings of the National Academy of Sciences (PNAS). DOI: https://doi.org/10.1073/pnas.1713139115. pdf
Recommended in F1000Prime as being of special significance in its field.
Supporting Information. pdf
Press Release(s):
- MIT Technology Review - Farmers seek to deploy powerful gene drive. pdf
- www.technlogyreview.de pdf (in german)
- UCSD News Center - Researchers Develop First Gene Drive Targeting Worldwide Crop Pest. pdf
- www.sciencemag.org - Can a genetic weapon combat one of the world’s major crop destroyers?. pdf
- www.kjzz.org - Researchers Turn to Gene Drive to Fight Destructive, Invasive Fruit Fly. pdf ; MP3
- https://igtrcn.org/gene-drive-in-a-major-agricultural-pest/ - Gene Drive in a Major Agricultural Pest. pdf
- Todays Science - Driving Out Pests with Genetic Engineering. pdf
- Farming Is Headed Toward A Gene-Editing Revolution, And Hoping The Public Comes Along, Too
- Editing Out Pesticides - Nova PBS
(29) Marshall, J.M*, Akbari, O.S.*, Can CRISPR-based gene drive be confined in the wild? A question for molecular and population biology. ACS Chemical Biology. 2018 Feb 7. doi: 10.1021/acschembio.7b00923. pdf.
bioRxiv 173914; doi: https://doi.org/10.1101/173914. pdf
Press Releases(s):
bioRxiv 173914; doi: https://doi.org/10.1101/173914. pdf
Press Releases(s):
(28) Vinauger, C., Lahondère1, C., Wolff, G.H., Locke, L.T., Liaw, J.E., Parrish, J.Z., Akbari, O.S., Dickinson, M.H., Riffell, J.A., Dopamine modulation of host learning in Aedes aegypti mosquitoes. (2018). Current Biology. doihttp://dx.doi.org/10.1016/j.cub.2017.12.015. pdf
bioRxiv. doi: https://doi.org/10.1101/172726. pdf
Insect Olfaction: Once Swatted, Twice Shy. Current Biology Dispatch. pdf
Press Releases(s):
bioRxiv. doi: https://doi.org/10.1101/172726. pdf
Insect Olfaction: Once Swatted, Twice Shy. Current Biology Dispatch. pdf
Press Releases(s):
- www.sciencenews.org .Rough lessons can lessen the pull of human scent on a mosquito. pdf
- www.nytimes.com. Swatting at Mosquitoes May Help You Avoid Bites, Even if You Miss. pdf
- www.nationalgeographic.com. First-Ever Evidence That Mosquitoes Can Be Trained. pdf
- www.washington.edu. If you swat mosquitoes, they may learn to avoid your scent. pdf
- www.popsci.com. Mosquitoes learn not to mess with you when you swat them. pdf
- www.nature.com. How to teach mosquitoes to avoid temptation. pdf
- www.npr.com. How To Teach Mosquitoes To Leave You Alone. pdf
2017
(27) Li M., Bui M., Yang T., White B.J., Akbari O.S.* Germline Cas9 Expression Yields Highly Efficient Genome Engineering in a Major Worldwide Disease Vector, Aedes aegypti. (2017). Proceedings of the National Academy of Sciences (PNAS) ; published ahead of print November 14, 2017, doi:10.1073/pnas.1711538114. pdf.
Supporting Information. pdf
PNAS Podcast.
Transcript PDF
Listen Here
Press Release(s):
Supporting Information. pdf
PNAS Podcast.
Transcript PDF
Listen Here
Press Release(s):
- IGTRCN - High Knock in/out Rates in Aedes aegypti Expressing Cas9 from a Transgene. pdf
- UCR Today - Making Mosquitoes Self-Destruct - UCR researchers are generating genetically engineered insects to help prevent the spread of infectious diseases. pdf
- www.wired.com - This gene-editing tech might be too dangerous to unleash. pdf
- www.sciencenews.org - Current CRISPR gene drives are too strong for outdoor use, studies warn. pdf
- www.geneengnews.com - Cas9 Expression in Mosquito Germlines Could Help Prevent Disease Spread. pdf
- www.ibtimes.com - Yellow, Three-Eyed, Wingless Mosquitoes Created Using CRISPR Gene Editing Tool.pdf
- www.news-medical.net - CRISPR shows potential to reduce diseases spread by mosquitoes. pdf
- www.entomologytoday.com - Another Step Forward in Genetic Engineering of Mosquitoes.pdf
- www.futurism.com - We Just Used Genetic Engineering to Create Completely Yellow, Three-Eyed, Wingless Mosquitoes
- PNAS - In this Issue. Cas9-expressing mosquito strains pave the way toward gene drives. pdf
(25) Marshall J.M.*, Buchman, A.B., Sanchez C. H.M., Akbari O.S.*, Overcoming evolved resistance to population-suppressing homing-based gene drives. Scientific Reports. (2017) doi:10.1038/s41598-017-02744-7. pdf
Supporting Information. pdf .
bioRxiv. (2016) doi: http://dx.doi.org/10.1101/088427. pdf
Press Release(s)
Supporting Information. pdf .
bioRxiv. (2016) doi: http://dx.doi.org/10.1101/088427. pdf
Press Release(s)
- Halting Mosquitoes on Continental Scale. pdf
- TheScientist-Researchers Propose Solution to Gene Drive Technology Problem. pdf
- New gene-editing technique could drive out mosquito borne disease. pdf
- UC Berkeley Press release. pdf
- www.geneengnews.com. pdf
- www.TheGuardian.ng.pdf
(24) Li M., Akbari O.S., White B.J., Highly efficient site-specific mutagenesis in Malaria mosquitoes using CRISPR. (2017). G3 (Bethesda). 2017 Dec 12. pii: g3.1134.2017. doi: 10.1534/g3.117.1134. pdf
Supporting Information. pdf
bioRxiv. pdf.
Press Release(s):
Supporting Information. pdf
bioRxiv. pdf.
Press Release(s):
(23) Li, M., Bui, M., Akbari, O.S.*, Embryo microinjection and transplantation technique for genome manipulation of Nasonia vitripennis. J. Vis. Exp., e56990, doi:10.3791/56990 (2017). pdf
Link to video here
Press Release(s):
Link to video here
Press Release(s):
(22) Li, M., Cook, L.U., Douglah, D., Chong, A., White, B.J., Ferree, P.M*, Akbari, O.S.*, Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9. Scientific Reports, Article number 901 (2017) doi:10.1038/s41598-017-00990-3. pdf
Supporting Information. pdf
bioRxiv Version here. pdf
Press Release(s) - Over 30 News Articles on this paper here are a few:
Radio/Video
BBC World Service Radio Interview - Listen Here
Youtube Video
Supporting Information. pdf
bioRxiv Version here. pdf
Press Release(s) - Over 30 News Articles on this paper here are a few:
- IGTRCN "Nasonia Genome Editing." pdf
- UCR Today "Researchers Create Red-eyed Mutant Wasps." pdf
- Gizmodo "Scientists Are Making Horrible Red-Eye Mutant Wasps Because Why the Hell Not." pdf
- UPI "Scientists engineer red-eyed mutant wasps." pdf
- Yahoo Tech. pdf
- SCI News. pdf
- www.iflscience.com. pdf
- Technology Review pdf
Radio/Video
BBC World Service Radio Interview - Listen Here
Youtube Video
2016
(20) H.M.T. Choi, C.R. Calvert, N. Husain, D. Huss, J.C. Barsi, B.E. Deverman, R.C. Hunter, M. Kato, S.M. Lee, A.C.T. Abelin, A.Z. Rosenthal, O.S. Akbari, Y. Li, B.A. Hay, P.W. Sternberg, P.H. Patterson, E.H. Davidson, S.K. Mazmanian, D.A. Prober, M. van de Rijn, J.R. Leadbetter, D.K. Newman, C. Readhead, M.E. Bronner, B. Wold, R. Lansford, T. Sauka-Spengler, S.E. Fraser, N.A. Pierce. Mapping a multiplexed zoo of mRNA expression. Development, (2016) 143, 3632-3637 doi:10.1242/dev.140137 . pdf
Press Releases(s):
Press Releases(s):
(19) Cheng B, Kuppanda N, Aldrich JC, Akbari OS*, Ferree PM*. Male-Killing Spiroplasma Alters Behavior of the Dosage Compensation Complex during Drosophila melanogaster Embryogenesis. Current Biology 2016 May 23;26(10):1339-45. doi: 10.1016/j.cub.2016.03.050. Epub 2016 May 5. PMID: 27161498. pdf
Primer Article
Host–Symbiont Interactions: Male-Killers Exposed. pdf
Press Release(s):
Primer Article
Host–Symbiont Interactions: Male-Killers Exposed. pdf
Press Release(s):
- Exploiting Male-Killing Bacteria to Control Insects. pdf
(18) Hall AB, Papathanos PA, Sharma A, Cheng C, Akbari OS, Assour L, Bergman NH, Cagnetti A, Crisanti A, Dottorini T, Fiorentini E, Galizi R, Hnath J, Jiang X, Koren S, Nolan T, Radune D, Sharakhova MV, Steele A, Timoshevskiy VA, Windbichler N, Zhang S, Hahn MW, Phillippy AM, Emrich SJ, Sharakhov IV, Tu ZJ, Besansky NJ. Radical remodeling of the Y chromosome in a recent radiation of malaria mosquitoes. Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):E2114-23. doi: 10.1073/pnas.1525164113. Epub 2016 Mar 29. PMID: 27035980. pdf
Press Release(s)
Press Release(s)
- Scientists Unlock Genetic Secret that Could Help Fight Malaria. pdf
(17) Champer J, Buchman A, Akbari OS*. Cheating evolution: engineering gene drives to manipulate the fate of wild populations. Nature Review Genetics. 2016 Mar;17(3):146-59. doi: 10.1038/nrg.2015.34. Epub 2016 Feb 15. Review. PMID: 26875679. pdf
Primer Article
Genetically-modified insects to combat vector-borne diseases — curtain rise or fall? pdf
Press Release(s):
Primer Article
Genetically-modified insects to combat vector-borne diseases — curtain rise or fall? pdf
Press Release(s):
- Engineered Gene Drives and the Future. pdf
- Insect Gene Drives – Reviewed and Compared. pdf
- PBS NOVA - For Gene Drives, Resistance May Be Inevitable
2015
(16) Marshall, J.M., Akbari, O.S., Chapter 9 - Gene Drive Strategies for Population Replacement. Pages 169-200 pdf In book Genetic Control of Malaria and Dengue, 1st Edition, Zach Adelman. 2015. ISBN: 978-0-12-800246-9. pdf.
(15) Ferree PM*, Fang C, Mastrodimos M, Hay BA, Amrhein H, Akbari OS*. Identification of Genes Uniquely Expressed in the Germ-Line Tissues of the Jewel Wasp Nasonia vitripennis. G3 (Bethesda). 2015 Oct 13;5(12):2647-53. doi: 10.1534/g3.115.021386.PMID: 26464360. Free PMC Article. pdf
Supplemental Information
Press Release(s):
Supplemental Information
Press Release(s):
- Entomologist’s Jewel Wasp Gene Research Published. pdf
:(14) Akbari OS, Bellen HJ, Bier E, Bullock SL, Burt A, Church GM, Cook KR, Duchek P, Edwards OR, Esvelt KM, Gantz VM, Golic KG, Gratz SJ, Harrison MM, Hayes KR, James AA, Kaufman TC, Knoblich J, Malik HS, Matthews KA, O'Connor-Giles KM, Parks AL, Perrimon N, Port F, Russell S, Ueda R, Wildonger J. Safeguarding gene drive experiments in the laboratory. Science. 2015 Aug 28;349(6251):927-9. doi: 10.1126/science.aac7932. Epub 2015 Jul 30. PMID: 26229113. pdf
Press Release(s):
Press Release(s):
(13) Li J, Olvera AI, Akbari OS, Moradian A, Sweredoski MJ, Hess S, Hay BA. Vectored antibody gene delivery mediates long-term contraception. Current Biology. 2015 Oct 5;25(19):R820-2. doi: 10.1016/j.cub.2015.08.002. PMID: 26439332. pdf
Supplemental Information
Press Release(s):
Supplemental Information
Press Release(s):
- Long-term contraception in a single shot. pdf
2014
(12) Akbari OS, Papathanos PA, Sandler JE, Kennedy K, Hay BA. Identification of germline transcriptional regulatory elements in Aedes aegypti. Scientific Reports. 2014 Feb 4;4:3954. doi: 10.1038/srep03954. PMID: 24492376. pdf
(11) Philippos A. Papathanos, Nikolai Windbichler and Omar S. Akbari. Sex Ratio Manipulation for Insect Population Control. Chapter 6. 2014.Page no: 83. pdf Transgenic Insects: Techniques and Applications. Mark Q. Benedict. 2014.
(10) Akbari OS*, Antoshechkin I, Hay BA, Ferree PM*. Transcriptome profiling of Nasonia vitripennis testis reveals novel transcripts expressed from the selfish B chromosome, paternal sex ratio. G3 (Bethesda). 2013 Sep 4;3(9):1597-605. dos: 10.1534/g3.113.007583. PMID: 23893741. pdf , Supplemental Data
(9) Akbari OS, Chen CH, Marshall JM, Huang H, Antoshechkin I, Hay BA. Novel synthetic Medea selfish genetic elements drive population replacement in Drosophila; a theoretical exploration of Medea-dependent population suppression. ACS Synthetic Biology. 2014 Dec 19;3(12):915-28. doi: 10.1021/sb300079h. Epub 2012 Dec 28. PMID: 23654248. pdf
2013
(8) Akbari OS, Antoshechkin I, Amrhein H, Williams B, Diloreto R, Sandler J, Hay BA. The developmental transcriptome of the mosquito Aedes aegypti, an invasive species and major arbovirus vector. G3 (Bethesda). 2013 Sep 4;3(9):1493-509. doi: 10.1534/g3.113.006742. PMID: 23833213. pdf
Supplemental Information
Online resource. www.vector.caltech.edu
Raw Data can be found at NCBI, Accession: PRJNA209388, Bioproject: 209388
Supplemental Information
Online resource. www.vector.caltech.edu
Raw Data can be found at NCBI, Accession: PRJNA209388, Bioproject: 209388
(7) Akbari OS, Matzen KD, Marshall JM, Huang H, Ward CM, Hay BA. A synthetic gene drive system for local, reversible modification and suppression of insect populations. Current Biology. 2013 Apr 22;23(8):671-7. doi: 10.1016/j.cub.2013.02.059. Epub 2013 Mar 28. PMID: 23541732. pdf
Supplemental Information
Primer Article
Supplemental Information
Primer Article
2011
(6) Ho MC, Schiller BJ, Akbari OS, Bae E, Drewell RA. Disruption of the abdominal-B promoter tethering element results in a loss of long-range enhancer-directed Hox gene expression in Drosophila. PLoS One. 2011 Jan 21;6(1):e16283. doi: 10.1371/journal.pone.0016283. Erratum in: PLoS One. 2011;6(2). doi: 10.1371/annotation/613079ca-aeae-4d29-bc5c-aa7202e6993e. PMID: 21283702. pdf
2010
(5) Oliver D, Sheehan B, South H, Akbari OS, Pai CY. The chromosomal association/dissociation of the chromatin insulator protein Cp190 of Drosophila melanogaster is mediated by the BTB/POZ domain and two acidic regions. BMC Cell Biology. 2010 Dec 31;11:101. doi: 10.1186/1471-2121-11-101. PMID: 21194420. pdf
2009
(4) Akbari OS, Oliver D, Eyer K, Pai CY. An Entry/Gateway cloning system for general expression of genes with molecular tags in Drosophila melanogaster. BMC Cell Biology. 2009 Jan 29;10:8. doi: 10.1186/1471-2121-10-8. PMID: 19178707. pdf
2008
(3) Akbari OS, Bae E, Johnsen H, Villaluz A, Wong D, Drewell RA. A novel promoter-tethering element regulates enhancer-driven gene expression at the bithorax complex in the Drosophila embryo. Development. 2008 Jan;135(1):123-31. Epub 2007 Nov 28. PMID: 18045839. pdf
2007
(2) Akbari OS, Schiller BJ, Goetz SE, Ho MC, Bae E, Drewell RA. The abdominal-B promoter tethering element mediates promoter-enhancer specificity at the Drosophila bithorax complex. Fly (Austin). 2007 Nov-Dec;1(6):337-9. PMID: 18504507. pdf
2006
(1) Akbari OS, Bousum A, Bae E, Drewell RA. Unraveling cis-regulatory mechanisms at the abdominal-A and Abdominal-B genes in the Drosophila bithorax complex. Developmental Biology. 2006 May 15;293(2):294-304. Epub 2006 Mar 20. Review. pdf
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